PrimePCR™ SYBR® Green Assay: PLG, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040727


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PrimePCR™ PreAmp for SYBR® Green Assay: PLG, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PLG, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038004
Assay Design:		exonic
Chromosome Location:		6:161127441-161127566question
Amplicon Length:		96
Splice Variants Targeted:		ENST00000419445 ENST00000379615 ENST00000358743 ENST00000347557 ENST00000333137 ENST00000404574 ENST00000353479 ENST00000369733 ENST00000396516 ENST00000355758 ENST00000366924 ENST00000308192 ENST00000418964 ENST00000355705 ENST00000409310 ENST00000359481

Gene Information

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Dec 2009]

Gene Symbol:		PLG
Gene Name:		plasminogen
Aliases:		DKFZp779M0222
RefSeq:		NC_000006.11 NG_016200.1 NT_025741.15
Ensembl:		ENSG00000122194
Entrez:		5340
Chromosome Mapping:		6q26

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.960000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.