PrimePCR™ SYBR® Green Assay: GGT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040765

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GGT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GGT2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038042
Assay Design:   exonic
Chromosome Location:   22:21563742-21567384question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000248923 ENST00000412658 ENST00000400382 ENST00000400380 ENST00000401885 ENST00000404532 ENST00000403838 ENST00000404223 ENST00000400383 ENST00000406383 ENST00000404912 ENST00000424787 ENST00000405188 ENST00000401924 ENST00000424627 ENST00000286890 ENST00000278765 ENST00000335694

Gene Information

GGT2 belongs to the gamma-glutamyltransferase (GGT; EC 2.3.2.2) gene family. GGT is a membrane-bound extracellular enzyme that cleaves gamma-glutamyl peptide bonds in glutathione and other peptides and transfers the gamma-glutamyl moiety to acceptors. GGT is also key to glutathione homeostasis because it provides substrates for glutathione synthesis (Heisterkamp et al. 2008 [PubMed 18357469]).[supplied by OMIM Oct 2008]

Gene Symbol:   GGT2
Gene Name:   gamma-glutamyltransferase 2
Aliases:   GGT, GGT 2
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000133475
Entrez:   728441
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995900
y-intercept 35.090000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download