PrimePCR™ SYBR® Green Assay: ZNF83, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040860

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ZNF83, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF83, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038137
Assay Design:   exonic
Chromosome Location:   19:53138319-53141574question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000421239 ENST00000601120 ENST00000596514 ENST00000391785 ENST00000301093 ENST00000593941 ENST00000540331 ENST00000600714 ENST00000601257 ENST00000597597 ENST00000545872 ENST00000596930 ENST00000597161 ENST00000598536 ENST00000544146 ENST00000536937 ENST00000301096 ENST00000457749 ENST00000391783 ENST00000596559 ENST00000594602 ENST00000414252 ENST00000595646 ENST00000243639 ENST00000597924 ENST00000396409 ENST00000390651 ENST00000597909 ENST00000601493 ENST00000597503 ENST00000243643 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF83
Gene Name:   zinc finger protein 83
Aliases:   FLJ11015, FLJ14876, FLJ30097, FLJ90585, HPF1, MGC33853, ZNF816B
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000167766
Entrez:   55769
Chromosome Mapping:   19q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 35.200000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download