PrimePCR™ SYBR® Green Assay: SFT2D3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040901

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SFT2D3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SFT2D3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038178
Assay Design:   exonic
Chromosome Location:   2:128459901-128460037question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000322313 ENST00000310981 ENST00000441917 ENST00000541887 ENST00000261819 ENST00000535482 ENST00000344395 ENST00000544314 ENST00000561603 ENST00000304330 ENST00000367588 ENST00000567887 ENST00000372915 ENST00000361689 ENST00000564288 ENST00000372925 ENST00000289893 ENST00000545844 ENST00000317713 ENST00000539005 ENST00000446571 ENST00000538716 ENST00000587989 ENST00000319511 ENST00000589785 ENST00000589856 ENST00000357984 ENST00000367222 ENST00000589140 ENST00000588045

Gene Information

Description Not Available

Gene Symbol:   SFT2D3
Gene Name:   SFT2 domain containing 3
Aliases:   MGC5391
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000173349
Entrez:   84826
Chromosome Mapping:   2q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.920000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download