PrimePCR™ SYBR® Green Assay: GPR150, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040925

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GPR150, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPR150, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038203
Assay Design:   exonic
Chromosome Location:   5:94955987-94956171question
Amplicon Length:   155
Splice Variants Targeted:   ENST00000339591 ENST00000449880 ENST00000450894 ENST00000200181 ENST00000579662 ENST00000310430 ENST00000518281 ENST00000373855 ENST00000373850 ENST00000373847 ENST00000431571 ENST00000238341 ENST00000519645 ENST00000341785 ENST00000333172 ENST00000351136 ENST00000411823 ENST00000328191 ENST00000380007 ENST00000261880 ENST00000561452 ENST00000199389 ENST00000295658 ENST00000406027 ENST00000454545

Gene Information

Description Not Available

Gene Symbol:   GPR150
Gene Name:   G protein-coupled receptor 150
Aliases:   PGR11
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000178015
Entrez:   285601
Chromosome Mapping:   5q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download