PrimePCR™ SYBR® Green Assay: PIGW, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040957

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PIGW, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PIGW, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038235
Assay Design:   exonic
Chromosome Location:   17:34893386-34893528question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000339282 ENST00000596193 ENST00000429467 ENST00000592983 ENST00000328396 ENST00000561548

Gene Information

Glycosylphosphatidylinositol (GPI) is a complex glycolipid that anchors many proteins to the cell surface. PIGW acts in the third step of GPI biosynthesis and acylates the inositol ring of phosphatidylinositol (Murakami et al. 2003 [PubMed 14517336]).[supplied by OMIM Mar 2008]

Gene Symbol:   PIGW
Gene Name:   phosphatidylinositol glycan anchor biosynthesis, class W
Aliases:   FLJ37433, Gwt1
RefSeq:   NC_000017.10 NT_010783.15 NW_003315949.1
Ensembl:   ENSG00000184886
Entrez:   284098
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.310000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download