PrimePCR™ SYBR® Green Assay: CLDN4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040987


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PrimePCR™ PreAmp for SYBR® Green Assay: CLDN4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CLDN4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038265
Assay Design:		exonic
Chromosome Location:		7:73245269-73245395question
Amplicon Length:		97
Splice Variants Targeted:		ENST00000321925 ENST00000587601 ENST00000586951 ENST00000415427 ENST00000402943 ENST00000435050 ENST00000431918 ENST00000340958 ENST00000398110 ENST00000421306 ENST00000262483 ENST00000272117 ENST00000429204 ENST00000573932 ENST00000574332 ENST00000573160

Gene Information

This gene encodes an integral membrane protein which belongs to the claudin family. The protein is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. This gene is deleted in Williams-Beuren syndrome a neurodevelopmental disorder affecting multiple systems. [provided by RefSeq Jul 2008]

Gene Symbol:		CLDN4
Gene Name:		claudin 4
Aliases:		CPE-R, CPER, CPETR, CPETR1, WBSCR8, hCPE-R
RefSeq:		NC_000007.13 NG_012868.1 NG_023289.1 NT_007933.15
Ensembl:		ENSG00000189143
Entrez:		1364
Chromosome Mapping:		7q11.23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	35.160000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.