PrimePCR™ SYBR® Green Assay: NOTCH2NL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0041082


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PrimePCR™ PreAmp for SYBR® Green Assay: NOTCH2NL, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NOTCH2NL, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038360
Assay Design:		exonic
Chromosome Location:		1:145281439-145281555question
Amplicon Length:		87
Splice Variants Targeted:		ENST00000394168 ENST00000314073 ENST00000356083 ENST00000245796 ENST00000441564 ENST00000295958 ENST00000325722 ENST00000426982 ENST00000341928 ENST00000342933 ENST00000256646 ENST00000602566 ENST00000362074 ENST00000344859 ENST00000369340 ENST00000598312 ENST00000358067 ENST00000486230 ENST00000320339 ENST00000371594 ENST00000536133 ENST00000544053 ENST00000451804 ENST00000406875 ENST00000428878 ENST00000342645 ENST00000581899 ENST00000602707 ENST00000578207 ENST00000594876 ENST00000593495 ENST

Gene Information

Description Not Available

Gene Symbol:		NOTCH2NL
Gene Name:		notch 2 N-terminal like
Aliases:		FLJ35032, FLJ55807, N2N
RefSeq:		NC_000001.10 NT_167185.1
Ensembl:		ENSG00000213240
Entrez:		388677
Chromosome Mapping:		1q21.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.520000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.