PrimePCR™ SYBR® Green Assay: KRTAP10-4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041101

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KRTAP10-4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KRTAP10-4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038379
Assay Design:   exonic
Chromosome Location:   21:45994755-45994972question
Amplicon Length:   188
Splice Variants Targeted:   ENST00000378330 ENST00000301030 ENST00000395358 ENST00000378623 ENST00000357503 ENST00000237536 ENST00000289547 ENST00000381160 ENST00000546276 ENST00000357474 ENST00000430587 ENST00000216214 ENST00000401533 ENST00000344651 ENST00000510168 ENST00000284674 ENST00000368608 ENST00000342740 ENST00000334420 ENST00000292579 ENST00000382303 ENST00000421177 ENST00000315691 ENST00000407192 ENST00000358994 ENST00000393370 ENST00000348141 ENST00000347048 ENST00000355007 ENST00000337738 ENST00000357197 ENST

Gene Information

This gene encodes a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur the ultrahigh sulfur and the high tyrosine/glycine KAPs. This gene encodes a member of the high sulfur KAP family. It is localized to a cluster of intronless KAPs at 21q22.3 which are located within the introns of the C21orf29 gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KRTAP10-4
Gene Name:   keratin associated protein 10-4
Aliases:   KAP10.4, KAP18-4, KRTAP10.4, KRTAP18-4, KRTAP18.4, MGC149545, MGC149546
RefSeq:   NC_000021.8 NT_011515.12
Ensembl:   ENSG00000215454
Entrez:   386672
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.100000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download