PrimePCR™ SYBR® Green Assay: KRTAP10-1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041102

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KRTAP10-1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KRTAP10-1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038380
Assay Design:   exonic
Chromosome Location:   21:45959587-45959718question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000359629 ENST00000537768 ENST00000380913 ENST00000418909 ENST00000393061 ENST00000507866 ENST00000323084 ENST00000400375 ENST00000400374 ENST00000400372 ENST00000397911 ENST00000334670 ENST00000375254 ENST00000375267 ENST00000375217 ENST00000375226

Gene Information

This gene encodes a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur the ultrahigh sulfur and the high tyrosine/glycine KAPs. This gene encodes a member of the high sulfur KAP family. It is localized to a cluster of intronless KAPs at 21q22.3 which are located within the introns of the C21orf29 gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KRTAP10-1
Gene Name:   keratin associated protein 10-1
Aliases:   KAP10.1, KAP18-1, KAP18.1, KRTAP10.1, KRTAP18-1, KRTAP18.1
RefSeq:   NC_000021.8 NT_011515.12
Ensembl:   ENSG00000215455
Entrez:   386677
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.940000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download