PrimePCR™ SYBR® Green Assay: POM121L4P, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041108

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: POM121L4P, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POM121L4P, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038386
Assay Design:   exonic
Chromosome Location:   22:21044734-21044855question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000542271 ENST00000356003 ENST00000448661 ENST00000568684 ENST00000359154 ENST00000427789 ENST00000301037 ENST00000530121 ENST00000409993 ENST00000354202 ENST00000432443 ENST00000412250 ENST00000329949 ENST00000394617 ENST00000320285 ENST00000366911 ENST00000332686

Gene Information

Description Not Available

Gene Symbol:   POM121L4P
Gene Name:   POM121 membrane glycoprotein-like 4 pseudogene
Aliases:   MGC119266
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000217261
Entrez:   266697
Chromosome Mapping:   22q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.720000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download