PrimePCR™ SYBR® Green Assay: TAPBP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041136

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TAPBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TAPBP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038414
Assay Design:   exonic
Chromosome Location:   6:33272939-33273034question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000388726 ENST00000392650 ENST00000575564 ENST00000389201 ENST00000599957 ENST00000358252 ENST00000532437 ENST00000374568 ENST00000374572 ENST00000395114 ENST00000456051 ENST00000552779 ENST00000550330 ENST00000412144 ENST00000383198 ENST00000383197 ENST00000451696 ENST00000550915 ENST00000549142 ENST00000262518 ENST00000395059 ENST00000344771 ENST00000337190 ENST00000263754 ENST00000367942 ENST00000372198 ENST00000447092 ENST00000373176 ENST00000290231 ENST00000434618 ENST00000475304 ENST

Gene Information

This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP) which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   TAPBP
Gene Name:   TAP binding protein (tapasin)
Aliases:   NGS17, TAPA, TPN, TPSN
RefSeq:   NC_000006.11 NG_009876.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000231925
Entrez:   6892
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.850000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download