PrimePCR™ SYBR® Green Assay: GALNT4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041191

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GALNT4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GALNT4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038469
Assay Design:   exonic
Chromosome Location:   12:89918007-89918102question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000548729 ENST00000529983 ENST00000202917 ENST00000445409

Gene Information

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain a stem region a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different but overlapping substrate specificities and patterns of expression. In vitro the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. Fusion transcripts which combine part of this gene with the 5' exons of the neighboring POC1B (POC1 centriolar protein homolog B) gene also exist. [provided by RefSeq Dec 2010]

Gene Symbol:   GALNT4
Gene Name:   UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)
Aliases:   FLJ93314, GALNAC-T4, GALNACT4
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000257594
Entrez:   8693
Chromosome Mapping:   12q21.33

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