PrimePCR™ SYBR® Green Assay: POU5F1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0041249


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PrimePCR™ PreAmp for SYBR® Green Assay: POU5F1B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: POU5F1B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038527
Assay Design:		exonic
Chromosome Location:		8:128427859-128428008question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000465342 ENST00000391675

Gene Information

This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1 however it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development. [provided by RefSeq Apr 2009]

Gene Symbol:		POU5F1B
Gene Name:		POU class 5 homeobox 1B
Aliases:		OCT4-PG1, OTF3C, OTF3P1, POU5F1P1, POU5F1P4, POU5FLC20, POU5FLC8
RefSeq:		NC_000008.10 NT_008046.16
Ensembl:		ENSG00000212993
Entrez:		5462
Chromosome Mapping:		8q24.21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.610000
Efficiency	92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.