PrimePCR™ SYBR® Green Assay: NDUFB4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041283

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NDUFB4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NDUFB4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038561
Assay Design:   exonic
Chromosome Location:   3:120315178-120315349question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000184266 ENST00000485064 ENST00000492739

Gene Information

This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq Dec 2009]

Gene Symbol:   NDUFB4
Gene Name:   NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
Aliases:   B15, CI-B15, MGC5105
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000065518
Entrez:   4710
Chromosome Mapping:   3q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.950000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download