PrimePCR™ SYBR® Green Assay: MAP1LC3B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041298

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAP1LC3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAP1LC3B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038576
Assay Design:   exonic
Chromosome Location:   16:87437885-87438001question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000268607 ENST00000534986

Gene Information

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP1LC3B
Gene Name:   microtubule-associated protein 1 light chain 3 beta
Aliases:   ATG8F, LC3B, MAP1A/1BLC3
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140941
Entrez:   81631
Chromosome Mapping:   16q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.170000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download