PrimePCR™ SYBR® Green Assay: FUZ, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041357

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FUZ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FUZ, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038635
Assay Design:   exonic
Chromosome Location:   19:50315912-50316264question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000349552 ENST00000435822 ENST00000528094 ENST00000533418 ENST00000313777 ENST00000529302 ENST00000526575 ENST00000445575 ENST00000389054 ENST00000520569 ENST00000518047 ENST00000398562 ENST00000389057 ENST00000398566 ENST00000398557 ENST00000253811 ENST00000354981 ENST00000408042 ENST00000355755 ENST00000378003 ENST00000557364 ENST00000399982 ENST00000263918 ENST00000379213 ENST00000504087 ENST00000344626 ENST00000429416 ENST00000541122 ENST00000589677 ENST00000444061 ENST00000590574 ENST

Gene Information

Description Not Available

Gene Symbol:   FUZ
Gene Name:   fuzzy homolog (Drosophila)
Aliases:   FLJ22688, FY
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000010361
Entrez:   80199
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.930000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download