PrimePCR™ SYBR® Green Assay: C1S, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0041489


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PrimePCR™ PreAmp for SYBR® Green Assay: C1S, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: C1S, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038767
Assay Design:		exonic
Chromosome Location:		12:7170194-7170299question
Amplicon Length:		76
Splice Variants Targeted:		ENST00000406697 ENST00000328916 ENST00000360817 ENST00000402681 ENST00000423384 ENST00000413211 ENST00000403949 ENST00000600933 ENST00000594877 ENST00000595575 ENST00000598620 ENST00000594871 ENST00000600357 ENST00000595846

Gene Information

This gene encodes a serine protease which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq Mar 2009]

Gene Symbol:		C1S
Gene Name:		complement component 1, s subcomponent
Aliases:		FLJ44757
RefSeq:		NC_000012.11 NG_011694.1 NT_009759.16
Ensembl:		ENSG00000182326
Entrez:		716
Chromosome Mapping:		12p13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998700
y-intercept	37.690000
Efficiency	90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.