PrimePCR™ SYBR® Green Assay: RP11-368J21.2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041552

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-368J21.2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-368J21.2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038830
Assay Design:   exonic
Chromosome Location:   16:22535156-22535265question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000524087 ENST00000415833 ENST00000543407 ENST00000541154 ENST00000538606 ENST00000517539 ENST00000528249 ENST00000424340 ENST00000520915 ENST00000568053 ENST00000550665 ENST00000504841 ENST00000419180 ENST00000521589 ENST00000534903 ENST00000448012 ENST00000458643 ENST00000341400 ENST00000518761 ENST00000543654 ENST00000415645 ENST00000357370 ENST00000451409 ENST00000399147 ENST00000547494 ENST00000538896 ENST00000526520 ENST00000531598 ENST00000552140 ENST00000530328 ENST00000529166 ENST

Gene Information

Description Not Available

Gene Symbol:   RP11-368J21.2
Gene Name:   Putative NPIP-like protein LOC100132247
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000243716
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.680000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download