PrimePCR™ SYBR® Green Assay: DCLRE1C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0041583


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PrimePCR™ PreAmp for SYBR® Green Assay: DCLRE1C, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: DCLRE1C, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038861
Assay Design:		exonic
Chromosome Location:		10:14995917-14996027question
Amplicon Length:		81
Splice Variants Targeted:		ENST00000378289 ENST00000378246 ENST00000357717 ENST00000378249 ENST00000396817 ENST00000378255 ENST00000378254 ENST00000378278 ENST00000378258 ENST00000418843 ENST00000378241 ENST00000456122 ENST00000453695

Gene Information

This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase DNA-activated catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq Jul 2008]

Gene Symbol:		DCLRE1C
Gene Name:		DNA cross-link repair 1C
Aliases:		A-SCID, DCLREC1C, FLJ11360, FLJ36438, RS-SCID, SCIDA, SNM1C, hSNM1C
RefSeq:		NC_000010.10 NG_007276.1 NT_008705.16
Ensembl:		ENSG00000152457
Entrez:		64421
Chromosome Mapping:		10p13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.560000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.