PrimePCR™ SYBR® Green Assay: MED24, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049215

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MED24, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MED24, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041952
Assay Design:   exonic
Chromosome Location:   17:38209726-38209851question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000394126 ENST00000356271 ENST00000394128 ENST00000394127 ENST00000501516 ENST00000428757 ENST00000543759 ENST00000585306 ENST00000537674 ENST00000582023 ENST00000580517 ENST00000578161 ENST00000580302 ENST00000536318 ENST00000431269 ENST00000369135 ENST00000332272 ENST00000319129 ENST00000389570 ENST00000592193 ENST00000586717 ENST00000581312

Gene Information

This gene encodes a component of the mediator complex (also known as TRAP SMCC DRIP or ARC) a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MED24
Gene Name:   mediator complex subunit 24
Aliases:   ARC100, CRSP100, CRSP4, DRIP100, KIAA0130, MGC8748, THRAP4, TRAP100, hTRAP100
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000008838
Entrez:   9862
Chromosome Mapping:   17q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.080000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download