PrimePCR™ SYBR® Green Assay: ATP2C1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049266

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP2C1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP2C1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042003
Assay Design:   exonic
Chromosome Location:   3:130711835-130712871question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000505330 ENST00000504381 ENST00000507488 ENST00000510168 ENST00000508532 ENST00000504948 ENST00000513801 ENST00000328560 ENST00000428331 ENST00000359644 ENST00000422190 ENST00000504612 ENST00000508660 ENST00000393221 ENST00000533801

Gene Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Aug 2011]

Gene Symbol:   ATP2C1
Gene Name:   ATPase, Ca++ transporting, type 2C, member 1
Aliases:   ATP2C1A, BCPM, HHD, KIAA1347, PMR1, SPCA1, hSPCA1
RefSeq:   NC_000003.11 NT_005612.16 NG_007379.1
Ensembl:   ENSG00000017260
Entrez:   27032
Chromosome Mapping:   3q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.490000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download