PrimePCR™ SYBR® Green Assay: SRCIN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049267


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PrimePCR™ PreAmp for SYBR® Green Assay: SRCIN1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SRCIN1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042004
Assay Design:		exonic
Chromosome Location:		17:36707515-36707604question
Amplicon Length:		60
Splice Variants Targeted:		ENST00000392619 ENST00000359323 ENST00000404898 ENST00000402142 ENST00000336649 ENST00000400164 ENST00000586406 ENST00000565365 ENST00000562477 ENST00000394048 ENST00000267113 ENST00000541590 ENST00000546954 ENST00000278903 ENST00000343678 ENST00000264659 ENST00000578925 ENST00000542707 ENST00000296452 ENST00000221347 ENST00000316005 ENST00000358965 ENST00000393695 ENST00000361256 ENST00000401886 ENST00000398042 ENST00000327374 ENST00000401833 ENST00000432883 ENST00000456048 ENST00000398633 ENST

Gene Information

Description Not Available

Gene Symbol:		SRCIN1
Gene Name:		SRC kinase signaling inhibitor 1
Aliases:		KIAA1684, P140, SNIP
RefSeq:		NC_000017.10 NT_010783.15 NW_003315948.1
Ensembl:		ENSG00000017373
Entrez:		80725
Chromosome Mapping:		17q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.440000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.