PrimePCR™ SYBR® Green Assay: SLC38A5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049268


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC38A5, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC38A5, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042005
Assay Design:		exonic
Chromosome Location:		X:48324469-48324706question
Amplicon Length:		72
Splice Variants Targeted:		ENST00000508239 ENST00000449771 ENST00000549151 ENST00000389212 ENST00000548919 ENST00000405493 ENST00000171000 ENST00000529022 ENST00000292524 ENST00000344063 ENST00000297788 ENST00000494552 ENST00000464672 ENST00000298532 ENST00000517309 ENST00000455950 ENST00000376876 ENST00000376875 ENST00000440085 ENST00000441948 ENST00000413668 ENST00000416711 ENST00000429543 ENST00000317669 ENST00000228606 ENST00000291574 ENST00000264741 ENST00000230124 ENST00000369451 ENST00000570156 ENST00000441106 ENST

Gene Information

The protein encoded by this gene is a system N sodium-coupled amino acid transporter involved in the transfer of glutamine asparagine histidine serine alanine and glycine. The encoded protein does not transport charged amino acids imino acids or N-alkylated amino acids. This transporter is not inhibited by lithium.[provided by RefSeq Jun 2010]

Gene Symbol:		SLC38A5
Gene Name:		solute carrier family 38, member 5
Aliases:		JM24, SN2, SNAT5, pp7194
RefSeq:		NC_000023.10 NG_021469.1 NT_079573.4
Ensembl:		ENSG00000017483
Entrez:		92745
Chromosome Mapping:		Xp11.23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.610000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.