PrimePCR™ SYBR® Green Assay: IKZF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049309


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PrimePCR™ PreAmp for SYBR® Green Assay: IKZF2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IKZF2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042046
Assay Design:		exonic
Chromosome Location:		2:213921817-214012529question
Amplicon Length:		75
Splice Variants Targeted:		ENST00000450892 ENST00000342002 ENST00000434687 ENST00000374319 ENST00000433134 ENST00000452786 ENST00000442445 ENST00000457361 ENST00000451136 ENST00000421754 ENST00000374327 ENST00000413091 ENST00000530649

Gene Information

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene but the biological validity of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:		IKZF2
Gene Name:		IKAROS family zinc finger 2 (Helios)
Aliases:		HELIOS, MGC34330, ZNF1A2, ZNFN1A2
RefSeq:		NC_000002.11 NT_005403.17
Ensembl:		ENSG00000030419
Entrez:		22807
Chromosome Mapping:		2q34

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	36.270000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.