PrimePCR™ SYBR® Green Assay: PI4K2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049335


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PrimePCR™ PreAmp for SYBR® Green Assay: PI4K2B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PI4K2B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042072
Assay Design:		exonic
Chromosome Location:		4:25278741-25278887question
Amplicon Length:		117
Splice Variants Targeted:		ENST00000512921 ENST00000264864

Gene Information

Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP) an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 45-bisphosphate synthesis (Wei et al. 2002 [PubMed 12324459]).[supplied by OMIM Jun 2008]

Gene Symbol:		PI4K2B
Gene Name:		phosphatidylinositol 4-kinase type 2 beta
Aliases:		FLJ11105, PI4KIIB, PIK42B
RefSeq:		NC_000004.11 NT_006316.16
Ensembl:		ENSG00000038210
Entrez:		55300
Chromosome Mapping:		4p15.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	35.670000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.