PrimePCR™ SYBR® Green Assay: LIMA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049390


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PrimePCR™ PreAmp for SYBR® Green Assay: LIMA1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: LIMA1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042127
Assay Design:		exonic
Chromosome Location:		12:50570948-50571073question
Amplicon Length:		96
Splice Variants Targeted:		ENST00000552491 ENST00000547825 ENST00000552823 ENST00000394943 ENST00000341247 ENST00000552783 ENST00000552909

Gene Information

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene and expression of some of the variants maybe independently regulated. [provided by RefSeq Aug 2011]

Gene Symbol:		LIMA1
Gene Name:		LIM domain and actin binding 1
Aliases:		EPLIN, FLJ38853, MGC131726, SREBP3
RefSeq:		NC_000012.11 NT_029419.12
Ensembl:		ENSG00000050405
Entrez:		51474
Chromosome Mapping:		12q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.996900
y-intercept	35.110000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.