PrimePCR™ SYBR® Green Assay: GALC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049416


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PrimePCR™ PreAmp for SYBR® Green Assay: GALC, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: GALC, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042153
Assay Design:		exonic
Chromosome Location:		14:88431956-88434807question
Amplicon Length:		117
Splice Variants Targeted:		ENST00000261304 ENST00000544807 ENST00000393569 ENST00000393568

Gene Information

This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide galactosylsphingosine lactosylceramide and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease also known as globoid cell leukodystrophy. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:		GALC
Gene Name:		galactosylceramidase
Aliases:		Not Available
RefSeq:		NC_000014.8 NG_011853.2 NT_026437.12
Ensembl:		ENSG00000054983
Entrez:		2581
Chromosome Mapping:		14q31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	36.000000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.