PrimePCR™ SYBR® Green Assay: BCS1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049614


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PrimePCR™ PreAmp for SYBR® Green Assay: BCS1L, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: BCS1L, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042351
Assay Design:		exonic
Chromosome Location:		2:219525687-219525786question
Amplicon Length:		70
Splice Variants Targeted:		ENST00000428880 ENST00000430322 ENST00000456050 ENST00000359273 ENST00000392109 ENST00000392110 ENST00000423377 ENST00000392111 ENST00000412366 ENST00000439945 ENST00000431802

Gene Information

This gene encodes a homolog of the S. cerevisiae bcs1 protein which is involved in the assembly of complex III of the mitochondrial respiratory chain. The encoded protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Five alternatively spliced transcripts encoding the same protein have been described. [provided by RefSeq Mar 2012]

Gene Symbol:		BCS1L
Gene Name:		BCS1-like (S. cerevisiae)
Aliases:		BCS, BCS1, BJS, FLNMS, GRACILE, Hs.6719, PTD, h-BCS
RefSeq:		NC_000002.11 NT_005403.17 NG_008018.1
Ensembl:		ENSG00000074582
Entrez:		617
Chromosome Mapping:		2q33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.550000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.