PrimePCR™ SYBR® Green Assay: SLC26A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049890


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC26A3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC26A3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042628
Assay Design:		exonic
Chromosome Location:		7:107434923-107443567question
Amplicon Length:		102
Splice Variants Targeted:		ENST00000340010 ENST00000453332 ENST00000422236

Gene Information

The protein encoded by this gene is a transmembrane glycoprotein that transports chloride ions across the cell membrane in exchange for bicarbonate ions. It is localized to the mucosa of the lower intestinal tract particularly to the apical membrane of columnar epithelium and some goblet cells. The protein is essential for intestinal chloride absorption and mutations in this gene have been associated with congenital chloride diarrhea. [provided by RefSeq Oct 2008]

Gene Symbol:		SLC26A3
Gene Name:		solute carrier family 26, member 3
Aliases:		CLD, DRA
RefSeq:		NC_000007.13 NG_008046.1 NT_007933.15
Ensembl:		ENSG00000091138
Entrez:		1811
Chromosome Mapping:		7q31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	37.310000
Efficiency	92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.