PrimePCR™ SYBR® Green Assay: SLC25A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0050003


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PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SLC25A1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042741
Assay Design:		exonic
Chromosome Location:		22:19163568-19163691question
Amplicon Length:		94
Splice Variants Targeted:		ENST00000458005 ENST00000215882 ENST00000451283 ENST00000335288 ENST00000394752 ENST00000358080 ENST00000296295 ENST00000394750 ENST00000598008 ENST00000599882

Gene Information

The mitochondrial tricarboxylate transporter (also called citrate transport protein or CTP) is responsible for the movement of citrate across the mitochondrial inner membrane (Kaplan et al. 1993 [PubMed 8514800]).[supplied by OMIM Jan 2011]

Gene Symbol:		SLC25A1
Gene Name:		solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Aliases:		CTP, SEA, SLC20A3
RefSeq:		NC_000022.10 NT_011519.10
Ensembl:		ENSG00000100075
Entrez:		6576
Chromosome Mapping:		22q11.21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.840000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.