PrimePCR™ SYBR® Green Assay: SDR39U1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050051

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SDR39U1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SDR39U1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042789
Assay Design:   exonic
Chromosome Location:   14:24909531-24909645question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000395715 ENST00000220888 ENST00000519076 ENST00000520276 ENST00000519674 ENST00000517323 ENST00000251343 ENST00000399395 ENST00000553930 ENST00000538105 ENST00000554698 ENST00000555365 ENST00000553343 ENST00000556249 ENST00000555225 ENST00000555355 ENST00000556523 ENST00000399390 ENST00000225964

Gene Information

Description Not Available

Gene Symbol:   SDR39U1
Gene Name:   short chain dehydrogenase/reductase family 39U, member 1
Aliases:   C14orf124, HCDI
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100445
Entrez:   56948
Chromosome Mapping:   14q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.470000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download