PrimePCR™ SYBR® Green Assay: UQCC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050102

List Price:    $188.00
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PrimePCR™ PreAmp for SYBR® Green Assay: UQCC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: UQCC, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042840
Assay Design:   exonic
Chromosome Location:   20:33902564-33935074question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000349714 ENST00000374384 ENST00000374380 ENST00000374385 ENST00000424405 ENST00000438533 ENST00000397554 ENST00000374377 ENST00000397556 ENST00000540457 ENST00000542501

Gene Information

This gene encodes a transmembrane protein that is structurally similar to the mouse basic fibroblast growth factor repressed ZIC-binding protein. In mouse this protein may be involved in fibroblast growth factor regulated growth control. In humans polymorphisms in this gene are associated with variation in human height and osteoarthritis. Alternate splicing results in multiple transcript variants. [provided by RefSeq May 2010]

Gene Symbol:   UQCC
Gene Name:   ubiquinol-cytochrome c reductase complex chaperone
Aliases:   BFZB, C20orf44, CBP3, MGC104353, MGC141902
RefSeq:   NC_000020.10 NT_011362.10 NG_021421.1
Ensembl:   ENSG00000101019
Entrez:   55245
Chromosome Mapping:   20q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download