PrimePCR™ SYBR® Green Assay: PLOD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050408

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLOD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLOD3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043146
Assay Design:   exonic
Chromosome Location:   7:100859815-100860455question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000409379 ENST00000397843 ENST00000532993 ENST00000356641 ENST00000569563 ENST00000347162 ENST00000259708 ENST00000479388 ENST00000394056 ENST00000394058 ENST00000440886 ENST00000370611 ENST00000553780 ENST00000554866 ENST00000554276 ENST00000329597 ENST00000223127 ENST00000414785 ENST00000456079 ENST00000312988 ENST00000321253

Gene Information

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq Jul 2008]

Gene Symbol:   PLOD3
Gene Name:   procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Aliases:   LH3
RefSeq:   NC_000007.13 NG_012148.1 NT_007933.15
Ensembl:   ENSG00000106397
Entrez:   8985
Chromosome Mapping:   7q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download