PrimePCR™ SYBR® Green Assay: EZH2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0050412


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PrimePCR™ PreAmp for SYBR® Green Assay: EZH2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EZH2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043150
Assay Design:		exonic
Chromosome Location:		7:148544300-148544391question
Amplicon Length:		62
Splice Variants Targeted:		ENST00000478654 ENST00000460911 ENST00000350995 ENST00000320356 ENST00000476773 ENST00000483967 ENST00000541220 ENST00000536783

Gene Information

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein the VAV1 oncoprotein and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:		EZH2
Gene Name:		enhancer of zeste homolog 2 (Drosophila)
Aliases:		ENX-1, ENX1, EZH1, KMT6, KMT6A, MGC9169
RefSeq:		NC_000007.13 NT_007914.15
Ensembl:		ENSG00000106462
Entrez:		2146
Chromosome Mapping:		7q35-q36

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	34.980000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.