PrimePCR™ SYBR® Green Assay: RAPGEF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0050446


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PrimePCR™ PreAmp for SYBR® Green Assay: RAPGEF1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RAPGEF1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043184
Assay Design:		exonic
Chromosome Location:		9:134526226-134585009question
Amplicon Length:		84
Splice Variants Targeted:		ENST00000372189 ENST00000372195 ENST00000438647 ENST00000427994 ENST00000372190

Gene Information

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis integrin-mediated signal transduction and cell transformation. Several alternatively spliced transcript variants of this gene have been described but the full-length nature of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:		RAPGEF1
Gene Name:		Rap guanine nucleotide exchange factor (GEF) 1
Aliases:		C3G, DKFZp781P1719, GRF2
RefSeq:		NC_000009.11 NT_035014.4
Ensembl:		ENSG00000107263
Entrez:		2889
Chromosome Mapping:		9q34.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.390000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.