PrimePCR™ SYBR® Green Assay: PRSS16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050721


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PrimePCR™ PreAmp for SYBR® Green Assay: PRSS16, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PRSS16, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043459
Assay Design:		exonic
Chromosome Location:		6:27220617-27222476question
Amplicon Length:		87
Splice Variants Targeted:		ENST00000421826 ENST00000230582 ENST00000485993 ENST00000475106 ENST00000535129

Gene Information

This gene encodes a serine protease expressed exclusively in the thymus. It is thought to play a role in the alternative antigen presenting pathway used by cortical thymic epithelial cells during the positive selection of T cells. The gene is found in the large histone gene cluster on chromosome 6 near the major histocompatibility complex (MHC) class I region. A second transcript variant has been described but its full length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:		PRSS16
Gene Name:		protease, serine, 16 (thymus)
Aliases:		FLJ36271, FLJ40714, FLJ44172, TSSP
RefSeq:		NC_000006.11 NT_007592.15
Ensembl:		ENSG00000112812
Entrez:		10279
Chromosome Mapping:		6p21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998400
y-intercept	34.990000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.