PrimePCR™ SYBR® Green Assay: ATP5F1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050945

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP5F1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP5F1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043683
Assay Design:   exonic
Chromosome Location:   1:111996959-111998812question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000369722 ENST00000483994

Gene Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core F1 and the membrane-spanning component Fo comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha beta gamma delta and epsilon) assembled with a stoichiometry of 3 alpha 3 beta and a single representative of the other 3. The proton channel seems to have nine subunits (a b c d e f g F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP5F1
Gene Name:   ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
Aliases:   MGC24431, PIG47
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000116459
Entrez:   515
Chromosome Mapping:   1p13.2

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