PrimePCR™ SYBR® Green Assay: TREH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051025

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TREH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TREH, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043763
Assay Design:   exonic
Chromosome Location:   11:118530535-118531248question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000529101 ENST00000530256 ENST00000525958 ENST00000264029 ENST00000397925 ENST00000598751 ENST00000602002

Gene Information

This gene encodes an enzyme that hydrolyses trehalose a disaccharide formed from two glucose molecules found mainly in fungi plants and insects. A partial duplication of this gene is located adjacent to this locus on chromosome 11. [provided by RefSeq Jul 2008]

Gene Symbol:   TREH
Gene Name:   trehalase (brush-border membrane glycoprotein)
Aliases:   MGC129621, TRE, TREA
RefSeq:   NC_000011.9 NG_023321.1 NT_033899.8
Ensembl:   ENSG00000118094
Entrez:   11181
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download