PrimePCR™ SYBR® Green Assay: CRHR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051102

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CRHR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CRHR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043840
Assay Design:   exonic
Chromosome Location:   17:43910834-43911103question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000550606 ENST00000548791 ENST00000552724 ENST00000551817 ENST00000314537 ENST00000352855 ENST00000577353 ENST00000398285 ENST00000580876 ENST00000339069 ENST00000293493

Gene Information

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress reproduction immune response and obesity. Alternative splicing results in multiple transcript variants one of which represents a read-through transcript with the neighboring gene MGC57346. [provided by RefSeq Jan 2012]

Gene Symbol:   CRHR1
Gene Name:   corticotropin releasing hormone receptor 1
Aliases:   CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R-1, CRH-R1, CRH-R1h, CRHR, CRHR1L, CRHR1f
RefSeq:   NC_000017.10 NG_009902.1 NT_010783.15 NT_167251.1
Ensembl:   ENSG00000120088
Entrez:   1394
Chromosome Mapping:   17q12-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download