PrimePCR™ SYBR® Green Assay: NAA60, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051201

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PrimePCR™ PreAmp for SYBR® Green Assay: NAA60, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NAA60, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043939
Assay Design:   exonic
Chromosome Location:   16:3529487-3529596question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000407558 ENST00000572169 ENST00000572757 ENST00000573593 ENST00000570372 ENST00000424546 ENST00000575733 ENST00000573201 ENST00000574950 ENST00000573580 ENST00000574328 ENST00000421765 ENST00000360862 ENST00000414063 ENST00000576787 ENST00000572942 ENST00000576916 ENST00000575076 ENST00000572131 ENST00000577013 ENST00000570819 ENST00000572584

Gene Information

Description Not Available

Gene Symbol:   NAA60
Gene Name:   N(alpha)-acetyltransferase 60, NatF catalytic subunit
Aliases:   FLJ11693, FLJ14154
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000122390
Entrez:   79903
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.130000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download