PrimePCR™ SYBR® Green Assay: ARFGEF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051282

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044020
Assay Design:   exonic
Chromosome Location:   20:47650859-47650968question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000371917

Gene Information

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq Jul 2008]

Gene Symbol:   ARFGEF2
Gene Name:   ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Aliases:   BIG2, FLJ23723, PVNH2, dJ1164I10.1
RefSeq:   NC_000020.10 NG_011490.1 NT_011362.10
Ensembl:   ENSG00000124198
Entrez:   10564
Chromosome Mapping:   20q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.040000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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