PrimePCR™ SYBR® Green Assay: ABCC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051340

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ABCC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ABCC4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044078
Assay Design:   exonic
Chromosome Location:   13:95838956-95839101question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000376887 ENST00000412704 ENST00000536256 ENST00000431522 ENST00000538287

Gene Information

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however this protein may play a role in cellular detoxification as a pump for its substrate organic anions. Alternative splicing results in multiple splice variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   ABCC4
Gene Name:   ATP-binding cassette, sub-family C (CFTR/MRP), member 4
Aliases:   EST170205, MOAT-B, MOATB, MRP4
RefSeq:   NC_000013.10 NT_009952.14
Ensembl:   ENSG00000125257
Entrez:   10257
Chromosome Mapping:   13q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.400000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download