PrimePCR™ SYBR® Green Assay: S1PR4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0051385


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PrimePCR™ PreAmp for SYBR® Green Assay: S1PR4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: S1PR4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044123
Assay Design:		exonic
Chromosome Location:		19:3179936-3180084question
Amplicon Length:		119
Splice Variants Targeted:		ENST00000246115 ENST00000373187 ENST00000356100 ENST00000373184 ENST00000373190 ENST00000373193 ENST00000373198 ENST00000373201 ENST00000514480

Gene Information

This gene is a member of the endothelial differentiation G-protein-coupled (EDG)) receptor gene family. EDG receptors bind lysophospholipids or lysosphingolipids as ligands and are involved in cell signalling in many different cell types. This EDG receptor gene is intronless and is specifically expressed in the lymphoid tissue. [provided by RefSeq Jul 2008]

Gene Symbol:		S1PR4
Gene Name:		sphingosine-1-phosphate receptor 4
Aliases:		EDG6, LPC1, S1P4, SLP4
RefSeq:		NC_000019.9 NT_011255.14
Ensembl:		ENSG00000125910
Entrez:		8698
Chromosome Mapping:		19p13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.660000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.