PrimePCR™ SYBR® Green Assay: RRAS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0051405


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PrimePCR™ PreAmp for SYBR® Green Assay: RRAS, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RRAS, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044143
Assay Design:		exonic
Chromosome Location:		19:50139983-50140179question
Amplicon Length:		71
Splice Variants Targeted:		ENST00000338074 ENST00000373946 ENST00000441639 ENST00000375401 ENST00000404049 ENST00000375379 ENST00000375383 ENST00000452825 ENST00000320345 ENST00000406424 ENST00000421807 ENST00000246792 ENST00000596526 ENST00000602020 ENST00000594714 ENST00000595211 ENST00000593469

Gene Information

Description Not Available

Gene Symbol:		RRAS
Gene Name:		related RAS viral (r-ras) oncogene homolog
Aliases:		Not Available
RefSeq:		NC_000019.9 NT_011109.16
Ensembl:		ENSG00000126458
Entrez:		6237
Chromosome Mapping:		19q13.3-qter

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	36.510000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.