PrimePCR™ SYBR® Green Assay: UBR4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051448


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PrimePCR™ PreAmp for SYBR® Green Assay: UBR4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: UBR4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044186
Assay Design:		exonic
Chromosome Location:		1:19401377-19403360question
Amplicon Length:		111
Splice Variants Targeted:		ENST00000375254 ENST00000375225 ENST00000375224 ENST00000375267 ENST00000375217 ENST00000375226 ENST00000543981 ENST00000429347

Gene Information

The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq Aug 2010]

Gene Symbol:		UBR4
Gene Name:		ubiquitin protein ligase E3 component n-recognin 4
Aliases:		FLJ41863, KIAA0462, KIAA1307, RBAF600, ZUBR1, p600
RefSeq:		NC_000001.10 NG_027669.1 NT_004610.19
Ensembl:		ENSG00000127481
Entrez:		23352
Chromosome Mapping:		1p36.13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	36.000000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.