PrimePCR™ SYBR® Green Assay: ZFP36, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051464

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZFP36, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZFP36, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044202
Assay Design:   exonic
Chromosome Location:   19:39898590-39898700question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000342537 ENST00000248673 ENST00000600033 ENST00000594442 ENST00000594045 ENST00000597629 ENST00000160740 ENST00000572681 ENST00000583376 ENST00000264039 ENST00000359139 ENST00000379370 ENST00000340305 ENST00000020673 ENST00000406432 ENST00000225308 ENST00000377095 ENST00000590194 ENST00000586016 ENST00000537904 ENST00000588767 ENST00000379902 ENST00000314276 ENST00000314250 ENST00000546602 ENST00000552570 ENST00000549700 ENST00000451358 ENST00000355730 ENST00000398879 ENST00000311160

Gene Information

Description Not Available

Gene Symbol:   ZFP36
Gene Name:   zinc finger protein 36, C3H type, homolog (mouse)
Aliases:   G0S24, GOS24, NUP475, RNF162A, TIS11, TTP
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000128016
Entrez:   7538
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download