PrimePCR™ SYBR® Green Assay: MPDU1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051521

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MPDU1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MPDU1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044259
Assay Design:   exonic
Chromosome Location:   17:7489289-7490028question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000250124 ENST00000396501 ENST00000584378 ENST00000423172 ENST00000579445 ENST00000585217 ENST00000581380 ENST00000396573 ENST00000562109 ENST00000330684 ENST00000396575 ENST00000404927 ENST00000535259

Gene Information

This gene encodes an endoplasmic reticulum membrane protein that is required for utilization of the mannose donor mannose-P-dolichol in the synthesis of lipid-linked oligosaccharides and glycosylphosphatidylinositols. Mutations in this gene result in congenital disorder of glycosylation type If. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2008]

Gene Symbol:   MPDU1
Gene Name:   mannose-P-dolichol utilization defect 1
Aliases:   CDGIF, FLJ14836, HBEBP2BPA, Lec35, My008, PP3958, PQLC5, SL15
RefSeq:   NC_000017.10 NG_009204.1 NT_010718.16
Ensembl:   ENSG00000129255
Entrez:   9526
Chromosome Mapping:   17p13.1-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download