This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051549
PrimePCR™ PreAmp for SYBR® Green Assay: LBP, Human
PrimePCR™ Template for SYBR® Green Assay: LBP, Human
The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid lipopolysaccharide (LPS) on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI) the encoded protein binds LPS and interacts with the CD14 receptor probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins including BPI plasma cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP). Finally this gene is found on chromosome 20 immediately downstream of the BPI gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.