PrimePCR™ SYBR® Green Assay: APOE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051559

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: APOE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APOE, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044297
Assay Design:   exonic
Chromosome Location:   19:45411108-45411198question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000532681 ENST00000332395 ENST00000245663 ENST00000395104 ENST00000356331 ENST00000442312 ENST00000503393 ENST00000374403 ENST00000374388 ENST00000316124 ENST00000333141 ENST00000007414 ENST00000392507 ENST00000278070 ENST00000370012 ENST00000413464 ENST00000369701 ENST00000369699 ENST00000486561 ENST00000274605 ENST00000348639 ENST00000326828 ENST00000367400 ENST00000252486 ENST00000446996 ENST00000434152 ENST00000425718 ENST00000409525 ENST00000360772 ENST00000346322 ENST00000475616 ENST

Gene Information

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E a main apoprotein of the chylomicron binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia or type III hyperlipoproteinemia (HLP III) in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq Jul 2008]

Gene Symbol:   APOE
Gene Name:   apolipoprotein E
Aliases:   AD2, LDLCQ5, LPG, MGC1571
RefSeq:   NC_000019.9 NG_007084.2 NT_011109.16
Ensembl:   ENSG00000130203
Entrez:   348
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.240000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download